Botox Injections can reduce the signs of ageing by reducing the muscle activity and wrinkles of the eyebrow and forehead. Botulinum toxin used in Botox injections is a sterile, purified protein produced from the bacteria, Clostridium botulinum, in the laboratory. Botox injected in small amounts into facial muscles binds to the nerve endings supplying the muscle and prevents nerve impulses from travelling to the muscle. When injected into muscle, it weakens or paralyses the muscle around the area injected for a typical period of several months. The resultant weakening or paralysing effect on the facial muscle temporarily reduces or eliminates previous skin creasing and new skin creasing is avoided. Botox may take several days to have its effect, and the effect typically lasts 3-6 months, in most individuals, before full muscle movement returns. At that point further treatment may be elected.